The urokinase chromogenic substrate is a high purity and high specificity urokinase chromogenic substrate synthesized through chemical methods, consisting of a small peptide with one end as a chromogenic group (pNA). Specific enzymes can catalyze the dissociation of pNA, and the free pNA can be detected using a spectrophotometer or a microplate reader, thus determining the activity of the corresponding substance.

The specific detection methods can adopt either dynamic or endpoint methods. The dynamic method involves continuously recording the changes in absorbance of the sample, calculating the change in absorbance per unit time, and reporting the results based on the change in absorbance per minute. The endpoint method refers to adding acetic acid to terminate the reaction after a certain period of time during which the active enzyme interacts with the chromogenic substance, detecting the change in absorbance during this period, and subsequently deducing the activity of the enzyme to be tested.

Stability: The lyophilized product that has not been reconstituted can be stored until the validity period indicated on the label.

After reconstitution: At room temperature (18-25℃), it can be stored for 7 days, and at 2-8℃, it can be stored for 3 months.