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Heparinase I can degrade heparin and heparan sulfate, making it a powerful tool for heparin structural research, clinical diagnosis, and industrial quality control.
Heparinase I selectively cleaves the linkages between glucosamine and O-sulfated iduronic acid in heparin and heparan sulfate (relative activity approximately 3:1), with the main products being disaccharides. This enzyme can also cleave the pentasaccharide binding site of antithrombin III within heparin molecules.
Company Name :
Beijing Asnail Biotechnology Co.LtdBrand :
AsnailFounded in 2017, Beijing Asrael Biotechnology Co., Ltd. has a business scope that includes the R&D and production of laboratory instrument consumables, animal blood products and product development in the diagnostic reagent industry.
In the direction of laboratory instrument consumables, for the three industrial directions of biotechnology and pharmaceutical industry, thrombosis and hemostasis industry and automotive polymer materials industry, Asrael screens out the corresponding combination of instruments and equipment, reagent consumables.
We adhere to the business philosophy of "professional first, honesty and trustworthiness", provide professional services and high-quality products for customers in related fields, shorten the process of customer procurement, improve efficiency, save costs, and strive to become a stable supplier trusted by customers.
Asrael's business objectives are:
We do not seek to be the largest in the industry, but rather to be the one that endures the longest.
We do not seek the fastest cooperation with clients, but rather the most enduring.
①Heparinases
Heparinases can clave glycosidic bonds of heparin and/or heparan sulfate by a β-elimination mechanism, generating unsaturated products (mostly
disaccharides) with a double bond be-tween C4 and C5 of the uronate residue.
Features:
● Natural heparinase, from Flavobacterium heparinum.
● It has the highest specific activity compare with other products on the market.
● Pure enzyme, free of BSA or other protein impurities.
● Good stability, consistent batch reproducibility and degradation characteristics.
②TOSOH TSKgel SWXL Columns
Tosoh SWxl series columns feature high porous volume/unit volume ratio, low sample absorption, and high column efficiency. Could be used for MW
determinations of heparin and LMWHs.
③BioCore™ SEC columns
Asnail co-developed the BioCore SEC-HP columns, tailored for molecular weight (MW) and MW distribution. The column is durable and can provide
better volume exclusion separation.
④Dionex™ IonPac™ AS11 IC Columns
The Column specifically designed to resolve a large number of inorganic anions and organicacid anions from a single sample injection in one gradient run using hydroxide eluent systems.USP officially specifies the method for the identification of heparin sodium chromatography.
| Place of origin | CHINA |
| Brand | Asnail |
| Item number | AS00-2519 |
| Usage | The raw materials for thromboelastography diagnostic reagents. |
| Packaging specifications | 0.1IU,1IU |
| CASNumber | 9025-39-2 |
| Purity | 99％ |
| Whether to import | No |
Characteristics of Heparinase I
Heparinase I produced by Beijing Asriel Biotechnology Co., Ltd. has the following characteristics:
Natural heparinase, derived from native heparin-degrading bacteria
Exhibits high specific activity (417 IU/mg)
Pure enzyme, free from BSA or other impurity proteins
Good stability, with consistent batch-to-batch reproducibility and degradation characteristics.
The form and composition of Heparinase I
Heparinase I is in solution form.
Main component: Heparinase I
Buffer solution: Phosphate buffer solution
The properties and specifications of Heparinase I
Name: Heparinase I; heparin lyase I; heparin eliminase I
Source: Flavobacterium heparinum
EC Number: 4.2.2.7
CAS Number: 9025-39-2
MDL Number: MFCD00131317
Molecular Weight: 42.8 kDa
Purity: ≥ 90% (HPLC)
Solubility: Soluble in pure water
Specific Activity: > 400 IU/mg
Concentration: 10 IU/mL
Mechanism of action, substrate specificity and unit definition of heparinase I
Mechanism of action of heparinase I: Heparinase selectively cleaves the α(1-4) glycosidic bond between glucosamine and uronic acid in sulfated heparin-glycans. This shearing process is accomplished by an elimination reaction to produce oligosaccharides containing unsaturated uronic acid residues (double bonds located between C4 and C5). The degradation products can be detected by UV spectroscopy (232 nm).
Substrate specificity of heparinase I: Heparin, heparan sulfate (relative activity approx. 3:1)
Definition of active units of heparinase I: 1 unit (1 IU) of heparinase I produces 1 μmol of unsaturated uronic acid per minute at pH 7.0 and 30 °C. 1 IU is approximately equal to 600 un (Sigma units).
How to use heparinase I
According to the application requirements, an appropriate amount of buffer solution was added to obtain the required concentration of heparinase I solution.
Stability and storage method of heparinase I
Heparinase I is stored at -20 °C in the dark, and the expiration date is about 12 months;
Leave the diluted heparinase I at -20 °C to prevent contamination.
Heparinase I is transported over long distances and requires the use of dry ice as a refrigerant.
Precautions for heparinase I
Heparinase I is suitable for the degradation of heparin and heparan sulfate and can only be used for laboratory scientific research, in vitro diagnostics and industrial quality control, and should not be used as a drug or in vivo diagnosis.
Heparinase application example
Heparinase has a wide range of scientific and industrial uses in scientific research and industry, such as:
Degrade heparin for structural analysis, such as enoxaparin sodium 1,6-ring formation rate test, heparin sodium disaccharide profiling
Heparin is partially degraded to obtain low molecular weight heparin, which is chemically modified or grafted to other materials
Degradation of crude heparin to detect its source (qPCR)
Degrade heparin and heparan sulfate to prepare heparin disaccharides and heparin oligosaccharides
Processing of plasma or other tissues for further study
Large-scale production of tinzaparin
Heparinase I, heparanase II, and heparinase III are commonly used together.
Heparinase I References
1. Linker, A., and Hovingh, P. Meth. Enzymol. 28, 902, (1972)
2. Lohse, D.L., and Linhardt, R.J. J. Biol. Chem. 267, 24347, (1992)
About heparin and heparan sulfate
Heparin and heparan sulfate are glycosaminoglycans (GAGs), which are linear polysaccharide mixtures linked by a repeating disaccharide unit composed of a uronic acid (D-glucuronic acid or L-iduronic acid) and a D-glucosamine/N-acetylglucosamine repeating disaccharide unit. Varying degrees of sulfation can occur on each monosaccharide residue, which can contain zero to three sulfate groups. In general, heparan is less sulfated than heparin.
Heparin and its derivative, low-molecular-weight heparin, are widely used anticoagulants. Heparinase plays an important role in the research and production of heparin and low molecular weight heparin.
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