Bioseparation technology is core to biotechnology, pharmaceuticals, and biomedical research. It separates, purifies, and identifies biomolecules like proteins, nucleic acids, and cells, being key for obtaining high-purity products.   Its key steps proceed sequentially harvesting (collecting cultures) clarification (removing impurities via centrifugation/filtration) concentration (increasing concentration through ultrafiltration/precipitation) purification (eliminating contaminants via chromatography/membrane separation) polishing (removing residual impurities with fine chromatography)   Technologies are classified by principle: Chromatography separates based on charge, specific binding, etc.; filtration/membrane separation includes ultrafiltration, microfiltration, and dialysis; electrophoresis covers capillary and gel types; centrifugation involves differential and density gradient methods.   Challenges include complex samples, molecule vulnerability to denaturation, and scaling difficulties, requiring parameter optimization and high-throughput screening.   Precise instruments like HPLC systems, centrifuges, filtration systems, and electrophoresis systems enhance separation speed and accuracy. As a foundation for high-quality biomolecules, it advances with technology.